Effects of Mounting a Breathable Membrane on the 384-Well Plate

Effects of Mounting a Breathable Membrane on the 384-Well Plate

A breathable membrane mounted on the top of the wells provides individual well seals and eliminates "edge effect" in evaporation.

Top: 96-well membrane-covered plate, room temperature, 28 days, no "edge effect" Middle: 384-well lid-covered plate, 37°C, 78% RH, 142 hours, obvious "edge effect" Bottom: 384-well lid-covered and membrane-mounted plate, same as middle, no "edge effect."

Top left: Lid-covered plate, in refrigerator; big condensate, cross-contamination Top right: lid- and membrane-covered plate, in refrigerator; fine condensate, no cross-contamination Bottom left: Lid- and membrane-covered plate, at 37°C, and 92% RH, no cross-contamination Bottom right: Lid-covered plate, at 37°C, and 92% RH; obvious contamination

Photos courtesy of Nalge Nunc International